Genetic engineering of porcine endothelial cell lines for ... - Nature
WEBJun 23, 2021 · We developed an efficient approach to quickly examine human-to-pig xeno-immune responses in vitro. A porcine endothelial cell was characterized and immortalized for genetic modification.
Establishment of porcine and human expanded potential stem cells - Nature
WEBJun 3, 2019 · Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in...
Porcine Macrophage Markers and Populations: An Update
WEBAug 19, 2023 · 4. Porcine Macrophage Cell Lines. Among various populations of porcine macrophages, PAM and monocyte-derived macrophages are the most frequently used in research studies on innate immune functions and host–pathogen interactions, because of their easy obtention.
Single-cell transcriptional profiling of porcine muscle satellite cells ...
WEBMar 1, 2024 · Porcine muscle satellite cells and myoblasts were characterized through single cell RNA-sequencing. New markers for porcine muscle satellite cells, including SDC2, SDC4, and ITGA7, were identified. ANGPTL4, a cytokine, inhibits porcine muscle satellite cell proliferation and differentiation.
An optimized culture system for efficient derivation of porcine ...
WEBMar 20, 2024 · Bona fide porcine pluripotent stem cells (PSCs) could facilitate testing cell and drug therapies. Agriculture and biotechnology may benefit from the ability to produce immune cells for...
The porcine innate immune system: An update - PMC
WEBApr 4, 2014 · Domestic pigs ( Sus scrofa domesticus) are closely related to humans in terms of their anatomy, genetics, and physiology, and often are the model of choice for the assessment of novel vaccines and therapeutics in a preclinical stage.
A novel method for isolation and culture of primary swine gastric ...
WEBJan 6, 2021 · Mechanical and enzymatic dissociation of gastric tissue was possible by combining collagenase type I and dispase II, protease inhibitors and antioxidants, which allowed the isolation of epithelial cells from the porcine fundic glands showing cell viability > 90% during the incubation period.
Validation of airway porcine epithelial cells as an alternative to ...
WEBSep 28, 2023 · In this paper, porcine and human tracheal and bronchial epithelial cells are compared in terms of in vivo tissue architecture and in vitro cell behaviour under standard and airlifted conditions, analyzing the regenerative, proliferative and differentiative potentials of …
Isolation, Culture and Identification of Porcine Skeletal Muscle ...
WEBAlthough the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited.
Biology of porcine T lymphocytes - PubMed
WEBThe development of porcine T cells begins in the liver during the first trimester of fetal life and continues in the thymus from the second trimester until after birth. Porcine T cells are divided into two lineages, based on their possession of the …
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