Zamore used both Solexa and 454 technologies to sequence short, noncoding RNAs from 5,000 fly heads. How do you isolate fly heads? Freeze the flies in liquid nitrogen and sift them with a flour sifter ...
RNA interference using small interfering RNAs (siRNAs) has become a mainstay of functional gene characterization and has generated over a dozen FDA-approved therapeutics and drugs in late-stage ...