Scientists first observed the biological phenomenon of restriction and modification in the 1950s, but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton ...

New England Biolabs' (NEB) popular web tools, Double Digest Finder and Enzyme Finder, are now available for the iPhone™ and iPod® Touch. These tools to help design restriction enzyme experiments are ...

The biological function of restriction enzymes is derived from the defense of foreign DNA from invading cells. The restriction-modification system of bacteria makes the DNA bases of bacteria to be ...

Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

Most restriction endonucleases use Mg²⁺ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that Bfil, a metal-independent restriction enzyme from the phospholipase D superfamily, ...

I remember clearly the first time I made a supervised trip, as an undergraduate student, to the departmental freezer to obtain a precious aliquot of restriction enzyme. Its real value, however, only ...

Studies have shown that restricting the intake of calories without reducing the intake of vitamins and minerals slows the signs of aging in a wide range of animals including monkeys, rats and fish, ...

"We are able to show that caloric restriction slows down aging by preventing an enzyme, peroxiredoxin, from being inactivated. This enzyme is also extremely important in counteracting damage to our ...

We describe the molecular cloning of the gal operon of Salmonella typhimurium LT2 and the localization of the gal promoter and the genes galE, galT, and galK. The order of the genes and the direction ...