A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)
Jul 21, 2022 · The FLIM method. Fluorescence lifetime imaging microscopy (FLIM) exploits the inherent properties of fluorescent dyes to produce an image. Fluorescent molecules have …
Fluorescence in Microscopy | Science Lab | Leica Microsystems
Apr 27, 2011 · Fluorescence in Microscopy. Fluorescence microscopy is a special form of light microscopy. It uses the ability of fluorochromes to emit light after being excited with light of a …
Introduction to Widefield Microscopy - Leica Microsystems
Jun 24, 2024 · Introduction to Widefield Microscopy. One of the basic microscopy techniques is known as ”widefield microscopy”. Fundamentally, widefield means the entire specimen or …
Fluorescent Dyes | Science Lab | Leica Microsystems
Jan 10, 2022 · A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – …
How to Prepare your Specimen for Immunofluorescence Microscopy
Jan 10, 2022 · Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy …
Total Internal Reflection Fluorescence (TIRF) Microscopy
Mar 11, 2012 · Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early …
Fluorescence | Applications - Leica Microsystems
Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy for its high sensitivity and high specificity. Integrated inverted digital microscopes …
An Introduction to Fluorescence | Science Lab - Leica Microsystems
Jun 2, 2023 · Fluorescence was first described by George Gabriel Stokes in 1852 [1]. He observed that fluorite glows after being illuminated with ultraviolet light. Fluorescence is a form …
Multicolor Microscopy: The Importance of Multiplexing
May 21, 2024 · Multicolor or multiplex microscopy is commonly used in research to better characterize disease and gain a better understanding of biological processes. For example, in …
Handbook of Optical Filters for Fluorescence Microscopy
Aug 6, 2013 · The Handbook of Optical Filters for Fluorescence Microscopy is a compilation of the principles that the engineers and scientists at Chroma Technology Corp. use to design filters …