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Understanding SYBR Green-Based qPCR
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gel electrophoresis is one of the most common molecular biology techniques. casting and running a gel is simple! combine TBE or TAE buffer with agarose to the desired concentration (usually 0.8-1% gel depending on size of DNA, so 40 mL buffer and 0.4 g agarose for a mini gel) as well as a dna stain like sybr safe. microwave until clear but do not let it boil over. let cool to around 55C then pour into a casting tray with a comb. once solid, the gel should be moved to the electrophoresis tank and
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